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J Oral Sci. 2019;61(2):284-293. doi: 10.2334/josnusd.18-0143.

Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets.

Author information

1
Department of Oral and Maxillofacial Surgery, Juntendo University Faculty of Medicine.
2
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry.
3
Department of Emergency and Critical Care Medicine, Keio University School of Medicine.
4
Department of Partial Denture Prosthodontics, Nihon University School of Dentistry.
5
Department of Oral Anatomy, Aichi-Gakuin University School of Dentistry.
6
Department of Clinical Medicine, Nihon University School of Dentistry.
7
Department of Life Science, Shimane University Faculty of Medicine.

Abstract

Bone marrow-derived mesenchymal stem cells (BMMSCs) remain the most widely used source of osteogenic cells in bone tissue engineering research. A cell-based treatment for alveolar ridge augmentation has received attention as an alternative to bone grafting. In the present study, BMMSC transplantation into tooth extraction sockets of C57BL/6J mice was evaluated for alveolar ridge regeneration. The first right maxillary molars were extracted, and then BMMSCs (PDGFRα+ Sca-1+ CD45- TER119- cells) isolated from femoral and tibial bone marrow were immediately transplanted into the extraction sockets. A control group underwent the same procedure except for BMMSC transplantation. Bone formation in the sockets was evaluated using micro-computed tomography and histological and immunohistochemical analyses. At 3 weeks, bone formation in the sockets was more advanced in the experimental group than in the control group. Histological analysis at 6 weeks after transplantation showed that the sockets in the experimental group also contained a greater quantity of bone marrow. Interestingly, socket bone mineral density was lower in the experimental group than in the control group at 6 weeks. These findings suggest that BMMSC transplantation accelerates bone healing and augments bone marrow formation in tooth extraction sockets.

KEYWORDS:

bone marrow; bone regeneration; dental socket; mesenchymal stem cells; tooth extraction

PMID:
31217377
DOI:
10.2334/josnusd.18-0143
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