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Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13563-13572. doi: 10.1073/pnas.1904280116. Epub 2019 Jun 19.

The purine biosynthesis regulator PurR moonlights as a virulence regulator in Staphylococcus aureus.

Author information

1
Department of Microbiology, New York University School of Medicine, New York, NY 10016.
2
Division of Infectious Diseases and Immunology, Department of Medicine, New York University School of Medicine, New York, NY 10016.
3
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
4
Proteomics Laboratory, New York University School of Medicine, New York, NY 10016.
5
Division of Advanced Research, New York University School of Medicine, New York, NY 10016.
6
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016.
7
Biomedical Hosting LLC, Arlington, MA 02474.
8
Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
9
Department of Microbiology, New York University School of Medicine, New York, NY 10016; victor.torres@nyulangone.org.

Abstract

The pathogen Staphylococcus aureus colonizes and infects a variety of different sites within the human body. To adapt to these different environments, S. aureus relies on a complex and finely tuned regulatory network. While some of these networks have been well-elucidated, the functions of more than 50% of the transcriptional regulators in S. aureus remain unexplored. Here, we assess the contribution of the LacI family of metabolic regulators to staphylococcal virulence. We found that inactivating the purine biosynthesis regulator purR resulted in a strain that was acutely virulent in bloodstream infection models in mice and in ex vivo models using primary human neutrophils. Remarkably, these enhanced pathogenic traits are independent of purine biosynthesis, as the purR mutant was still highly virulent in the presence of mutations that disrupt PurR's canonical role. Through the use of transcriptomics coupled with proteomics, we revealed that a number of virulence factors are differentially regulated in the absence of purR Indeed, we demonstrate that PurR directly binds to the promoters of genes encoding virulence factors and to master regulators of virulence. These results guided us into further ex vivo and in vivo studies, where we discovered that S. aureus toxins drive the death of human phagocytes and mice, whereas the surface adhesin FnbA contributes to the increased bacterial burden observed in the purR mutant. Thus, S. aureus repurposes a metabolic regulator to directly control the expression of virulence factors, and by doing so, tempers its pathogenesis.

KEYWORDS:

MRSA; PurR; infection; pathogenesis; purine biosynthesis

PMID:
31217288
PMCID:
PMC6613142
[Available on 2019-12-19]
DOI:
10.1073/pnas.1904280116

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