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J Glob Antimicrob Resist. 2019 Jun 16. pii: S2213-7165(19)30151-1. doi: 10.1016/j.jgar.2019.06.007. [Epub ahead of print]

Genomic diversification of IncR plasmids from China.

Author information

1
Medical Laboratory Center, General Hospital of People's Liberation Army, Beijing, 100085, China; Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: 527173138@qq.com.
2
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: 476115657@qq.com.
3
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: jerry9yin@163.com.
4
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: huling03@163.com.
5
Medical Laboratory Center, General Hospital of People's Liberation Army, Beijing, 100085, China. Electronic address: cherryzju@aliyun.com.
6
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: fionyoung@163.com.
7
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: 30235922@qq.com.
8
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: gb5029@hotmail.com.
9
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: zhaoyuee163@163.com.
10
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: dongshengzhou1977@gmail.com.
11
Medical Laboratory Center, General Hospital of People's Liberation Army, Beijing, 100085, China; Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: wangcb301@126.com.
12
Medical Laboratory Center, General Hospital of People's Liberation Army, Beijing, 100085, China. Electronic address: ypluo301@yeah.net.

Abstract

OBJECTIVES:

This study dealt with a detailed genomic characterization of IncR plasmids from China.

METHODS:

Three IncR plasmids p13190-tetA, p02085-tetA and p30860-tetA from clinical isolates of Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter freundii were fully sequenced by high-throughput genome sequencing, and then compared with five previously sequenced IncR plasmids pHN84KPC, pSH-01, pK245, pKPC_P16, and pKPC-LK30.

RESULTS:

These eight IncR plasmids from China possessed conserved IncR backbones composed of repB, parAB, umuCD, retA and resD. Resistance accessory modules integrated into IncR backbones included the MDR regions in p30860-tetA, p02085-tetA, p13190-tetA and pK245, the blaKPC-2 regions in pHN84KPC, pKPC-LK30 and pKPC_P16, and the ΔTn1721-sil region in pSH-01. These resistance accessory modules were inserted at a site between retA and vagD, resulting in loss of backbone genes vagCD in some of these plasmids, and they differed dramatically from one another and carried distinct profiles of resistance makers. In particular, all of p13190-tetA, p02085-tetA and p30860-tetA, and pHN84KPC, pSH-01 and pK245 carried tetracycline-resistance tet gene modules and the carbapenemase gene blaKPC-2 was identified in pHN84KPC, pKPC-LK30 and pKPC_P16. In addition, one or more regions responsible for plasmid replication and/or maintenance were found in some of these resistance accessory modules, facilitating stable replication of corresponding IncR plasmids at steady-state copy numbers.

CONCLUSIONS:

This is a detailed comparative genomics analysis of IncR plasmids from China, providing a deeper insight into diversification and evolution of IncR plasmids.

KEYWORDS:

IncR plasmids; bla(KPC); drug resistance; mobile elements; tetA

PMID:
31216492
DOI:
10.1016/j.jgar.2019.06.007

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