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Int J Mol Sci. 2019 Jun 12;20(12). pii: E2869. doi: 10.3390/ijms20122869.

Metabolomics Studies on Cytoplasmic Male Sterility during Flower Bud Development in Soybean.

Author information

1
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. xlding2012@njau.edu.cn.
2
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. 2014101141@njau.edu.cn.
3
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. 2017101147@njau.edu.cn.
4
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. 2017101148@njau.edu.cn.
5
Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, USDA-ARS, Beltsville, MD 20705, USA. Qijian.Song@ARS.USDA.GOV.
6
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. sri@njau.edu.cn.
7
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. spyang@njau.edu.cn.

Abstract

Abnormal reactive oxygen species (ROS) may mediate cytoplasmic male sterility (CMS). To observe the effect of ROS on soybean CMS, metabolite content and antioxidant enzyme activity in the flower buds between soybean N8855-derived CMS line and its maintainer were compared. Of the 612 metabolites identified, a total of 74 metabolites were significantly differentiated in flower buds between CMS line and its maintainer. The differential metabolites involved 32 differential flavonoids, 13 differential phenolamides, and 1 differential oxidized glutathione (GSSG) belonging to a non-enzymatic ROS scavenging system. We observed lower levels of flavonoids and antioxidant enzyme activities in flower buds of the CMS line than in its maintainer. Our results suggest that deficiencies of enzymatic and non-enzymatic ROS scavenging systems in soybean CMS line cannot eliminate ROS in anthers effectively, excessive accumulation of ROS triggered programmed cell death and ultimately resulted in pollen abortion of soybean CMS line.

KEYWORDS:

enzymatic ROS scavenging system; flower bud development; metabolomics; non-enzymatic ROS scavenging system; reactive oxygen species; soybean CMS-N8855

PMID:
31212804
DOI:
10.3390/ijms20122869
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