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Artif Organs. 2019 Jun 18. doi: 10.1111/aor.13518. [Epub ahead of print]

Teburu-Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models.

Author information

1
Department of Surgery, Campus Charité Mitte I Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
2
Cluster of Excellence, Interdisciplinary Laboratory Image Knowledge Gestaltung, Humboldt-Universität zu Berlin, Berlin, Germany.
3
Biofluid Machanics Laboratory, Institute for Imaging Science and Computational Modelling in Cardiovascular Medicine, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
4
Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
5
Institute for Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.

Abstract

Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.

KEYWORDS:

3D cell culture; 3D-printing; bioreactor; optogenetics; recellularization

PMID:
31211867
DOI:
10.1111/aor.13518

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