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J Tissue Eng. 2019 May 31;10:2041731419852703. doi: 10.1177/2041731419852703. eCollection 2019 Jan-Dec.

Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

Author information

1
Centre for Clinical Dental Research, Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
2
Department of Biomedicine, University of Bergen, Bergen, Norway.
3
Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
4
Cardiff Institute for Tissue Engineering and Repair (CITER), Cardiff, UK.
5
Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, Sweden.

Abstract

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

KEYWORDS:

Growth factor; bone tissue engineering; drug delivery; functionalisation; mesenchymal stem cell

Conflict of interest statement

Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.

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