Send to

Choose Destination
J Tissue Eng. 2019 May 31;10:2041731419852703. doi: 10.1177/2041731419852703. eCollection 2019 Jan-Dec.

Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration.

Author information

Centre for Clinical Dental Research, Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
Department of Biomedicine, University of Bergen, Bergen, Norway.
Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
Cardiff Institute for Tissue Engineering and Repair (CITER), Cardiff, UK.
Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, Sweden.


Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.


Growth factor; bone tissue engineering; drug delivery; functionalisation; mesenchymal stem cell

Conflict of interest statement

Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center