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Electrophoresis. 2019 Jun 17. doi: 10.1002/elps.201900045. [Epub ahead of print]

Development and validation of a novel SiFaSTRTM 23-plex system.

Zheng H1,2, Tao R2,3, Zhang J1,2, Zhang J1,2, Wang S3, Yang Z2,4, Xu Q2,4, Gao Y1, Zhang S2, Li C1,2.

Author information

1
Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China.
2
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Shanghai, P. R. China.
3
Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, P. R. China.
4
Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, P. R. China.

Abstract

Human identification and paternity testing are usually based on the study of STRs depending on their particular characteristics in the forensic investigation. In this paper, we developed a sensitive genotyping system, SiFaSTR 23-plex, which is able to characterize 18 expanded Combined DNA Index System STRs (D3S1358, D5S818, D2S1338, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D10S1248, D8S1179, D1S1656, D18S51, D12S391, D19S433, D16S539, D13S317, and FGA), three highly polymorphic STRs among Chinese people (Penta D, Penta E, and D6S1043), one Y-chromosome Indel and amelogenin using a multiplex PCR; the PCR amplified products were analyzed using the Applied Biosystems 3500 Genetic Analyzer. Full genotyping profiles were obtained using only 31.25 pg of control DNA; various case-type specimens, as well as ten-year-old samples were also successfully genotyped. Additionally, in the validation studies, this multiplex was demonstrated to be human-specific and compatible with the interference of multiple PCR inhibitors. The system also enabled the detection of mixtures, and complete profiles could be obtained at the mixed ratios of 1:1, 1:3, and 3:1. The development and validation study here illustrated that the SiFaSTR 23-plex system is accurate, powerful, and more sensitive than many other systems. What's more, the population data in our study not only illustrated that this 23-plex typing system was straightforward and efficient but also expanded the Chinese STR database, which could facilitate the appropriate application of the 23 genetic markers in forensic genetics, especially in the Chinese population.

KEYWORDS:

23-plex multiplex; Capillary electrophoresis; DNA genotyping; Developmental validation; Forensic science

PMID:
31209888
DOI:
10.1002/elps.201900045

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