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Biochem Biophys Res Commun. 2019 Aug 13;516(1):222-228. doi: 10.1016/j.bbrc.2019.05.176. Epub 2019 Jun 14.

Differentiation of lymphoblastoid-derived iPSCs into functional cardiomyocytes, neurons and myoblasts.

Author information

1
CERVO Brain Research Center, Institut Universitaire en santé mentale de Québec, Quebec City, QC, G1J 2G3, Canada.
2
Human Genetics Unit, Laboratoire d'Organogénèse Experimentale (LOEX). Hôpital de l'Enfant-Jésus, CHU Research Center, Quebec City, QC, G1J 1Z4, Canada.
3
Human Genetics Unit, Laboratoire d'Organogénèse Experimentale (LOEX). Hôpital de l'Enfant-Jésus, CHU Research Center, Quebec City, QC, G1J 1Z4, Canada; Department of Medicine, Université Laval, Quebec City, QC, G1K 7P4, Canada.
4
CERVO Brain Research Center, Institut Universitaire en santé mentale de Québec, Quebec City, QC, G1J 2G3, Canada; Department of Medicine, Université Laval, Quebec City, QC, G1K 7P4, Canada. Electronic address: mohamed.chahine@phc.ulaval.ca.

Abstract

Human induced pluripotent stem cells (hiPSCs) are a valuable tool for investigating complex cellular and molecular events that occur in several human diseases. Importantly, the ability to differentiate hiPSCs into any human cell type provides a unique way for investigating disease mechanisms such as complex mental health diseases. The in vitro transformation of human lymphocytes into lymphoblasts (LCLs) using the Epstein-Barr virus (EBV) has been the main method for generating immortalized human cell lines for half a century. However, the derivation of iPSCs from LCLs has emerged as an alternative source from which these cell lines can be generated. We show that iPSCs derived from LCLs using the Sendai virus procedure can be successfully differentiated into cardiomyocytes, neurons, and myotubes that express neuron- and myocyte-specific markers. We further show that these cardiac and neuronal cells are functional and generate action potentials that are required for cell excitability. We conclude that the ability to differentiate LCLs into neurons and myocytes will increase the use of LCLs in the future as a potential source of cells for modelling a number of diseases.

KEYWORDS:

Cardiac; Differentiation; Electrophysiology; Lymphoblast; Myoblasts; Neuronal; Pluripotent stem cells; Stem cells; iPSCs

PMID:
31208718
DOI:
10.1016/j.bbrc.2019.05.176

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