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Andrologia. 2019 Jun 17:e13344. doi: 10.1111/and.13344. [Epub ahead of print]

Effect of low androgen status on the expression of adenosine A2A and A2B receptors in rat penile corpus cavernosum.

Author information

1
Department of Urology, Nephropathy Clinical Medical Research Center of Sichuan Province, Affiliated Hospital, Southwest medical University, Luzhou, China.
2
Department of Thyroid Surgery, Affiliated Hospital, Southwest Medical University, Luzhou, China.

Abstract

To investigate whether low androgen status affects erectile function by regulating the expression of adenosine A2A and A2B receptors in rat penile corpus cavernosum. Thirty-six 8-week-old male Sprague-Dawley rats were randomly divided into six groups: sham-operated group (4w-sham, 8w-sham), castration group (4w-cast, 8w-cast) and androgen replacement group (4w-cast+T, 8w-cast+T). The rats in the androgen replacement groups were subcutaneously injected with testosterone propionate (3 mg/kg) every other day after castration. The maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP), the expression of A2A , A2B , AKT and eNOS and the concentrations of cAMP and cGMP in the corpus cavernosum were detected at the 4th and 8th weeks after the operation. The serum testosterone level and the ratio of ICPmax/MAP decreased significantly in the castration group as compared to other groups (p < 0.01). There was no significant difference in the expression of A2A receptor among groups, while the expression of A2B , AKT and eNOS and the concentrations of cAMP and cGMP in the castration group were significantly lower than in other groups (p < 0.01). Low androgen status inhibits the AKT/eNOS/cGMP signalling pathways and the production of cAMP in the corpus cavernosum of castrated rats by down-regulating the expression of A2B receptor, and results in decreased of ICPmax/MAP.

KEYWORDS:

adenosine receptors; androgen; eNOS; erectile dysfunction; rat

PMID:
31206753
DOI:
10.1111/and.13344

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