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J Food Sci. 2019 Jun 17. doi: 10.1111/1750-3841.14657. [Epub ahead of print]

Analysis of Young Shoots of 'Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics.

Zeng C1,2,3,4, Lin H4, Liu Z1,2,3, Liu Z4,5.

Author information

1
College of Life Science and Technology, Central South Univ. of Forestry and Technology, Changsha, Hunan, 410004, China.
2
Hunan Provincial Base for Scientific and Technological Innovation Cooperation, Changsha, 410004, China.
3
Hunan Provincial Key Lab. of Crop Germplasm Innovation and Utilization, Changsha, 410128, China.
4
Key Lab. of Tea Science, Ministry of Education, Hunan Agricultural Univ., Changsha, Hunan, 410128, China.
5
Natl. Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, Hunan, 410128, China.

Abstract

'Anji Baicha' (Camellia sinensis) is a low-temperature-sensitive tea variety. During the development of young shoots, the leaves of 'Anji Baicha' exhibit periodic albinism. The quality of 'Anji Baicha' is closely related to the color of the fresh leaves, with whiter leaves affording a higher amino acid content and superior sensory quality after processing. However, the metabolic mechanism of its quality formation is still unclear. In this study, we analyzed the metabolomic changes of young shoots of 'Anji Baicha' and screened for metabolic markers that may be involved in the periodic albinism. Positive- and negative-mode UPLC-QTOF-MS was applied to the metabolomic analysis of young leaves of 'Anji Baicha' during three developmental stages (i.e., the pre-albescent, albescent, and regreening stages). The results revealed significant differences in the metabolic profiles of the young leaves at the three stages. The differential metabolites were mainly related to the pathways of flavonoid, phenylpropanoid, and amino acid biosynthesis. The concentrations of several amino acids (primarily l-theanine, l-glutamate, N2 -acetyl-l-ornithine, l-aspartic acid, d-proline, l-glutamine, l-leucine, and pyroglutamic acid) and 12-OPDA were significantly higher in the albescent stage. In contrast, during the albescent stages, the concentrations of several carbohydrates (d-fructose, β-d-galactopyranose, 3-O-fucopyranosyl-2-acetamido-2-deoxyglucopyranose, galactose-β-1, 4-xylose acetyl-maltose, and 2-fucosyllactose) were significantly lower. Moreover, catechins (mainly epigallocatechin and catechin derivatives), dimeric catechins (primarily proanthocyanidins), and flavonol and flavonol/flavone glycosides (mainly kaempferol, myricetin, quercetin, cyanidin, and delphinidin glycosides) were detected at the highest levels in the regreening or pre-albescent stages. The obtained results enhance the current understanding of the metabolic mechanisms of periodic albinism and quality development formation in 'Anji Baicha'. PRACTICAL APPLICATION: The obtained results not only provide information regarding differential metabolites but also advance the understanding of the mechanism of periodic albinism in 'Anji Baicha' at the metabolite level and open up new possibilities for the genetic improvement of tea cultivars.

KEYWORDS:

LC-MS; chemical components; developmental stages; metabolomics; ‘Anji Baicha’ (Camellia sinensis)

PMID:
31206686
DOI:
10.1111/1750-3841.14657

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