Format

Send to

Choose Destination
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Jun;27(3):893-898. doi: 10.19746/j.cnki.issn.1009-2137.2019.03.041.

[Analysis of Gene Mutation Types of Thalassemia in Fuzhou Area of China].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Department of Clinical Laborabtorial Examination, Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350004, Fujian province, China.
2
Department of Clinical Laboratorial Examination, Fujian Provincial Hospital,Fuzhou 350001, Fujian province, China.
3
Department of Clinical Laborabtorial Examination, College of Integrated traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China.
4
Department of Clinical Laboratorial Examination, Fujian Provincial Hospital,Fuzhou 350001, Fujian province, China,E-mail: falinchen@126.com.

Abstract

in English, Chinese

OBJECTIVE:

To investigate the gene mutation types and spectrum of α, β-thalassemia in Fuzhou area of China.

METHODS:

Thalassemia gene screening was performed in the women receiving physical, prenatal, and pre-pregnancy examination, and the patients with suspected thalassemia in our hospital from July 2013 to March 2018.Genotypes of thalassem were detected by Gap-PCR and RDB-PCR.

RESULTS:

1042 were positive among 2074 suspected cases with a positive rate of 50.24%; 618 cases were confirmed to be α-thalassemia and with a positive rate of 29.8%; 409 cases were confirmed to be β-thalassemia with a positive rate of 19.72%. 15 cases were confirmed to be α-β complex thalassemia with a positive rate of 0.72%. the --SEA/αα(76.54%) was the most common genotype among α-thalassemia, -α3.7/αα(10.03%) and -α4.2/αα(2.91%) in hot pursuit. In addition, IVS-II-55 (T->G) and IVS-II-119 (-G, +CTCGGCCC) were newly found alpha mutations; the IVS-2-654 (C→T) (40.83%) was the most common genotype among β-thalassemia, CD41-42 (-TCTT) (35.94%) and CD17 (A→T) (9.78%) in hot pursuit.

CONCLUSION:

The genotype of thalassemia in Fuzhou area is highly heterogenic, --SEA/αα is the most common genotype among α-thalassemia, IVS-2-654 (C→T) is the most common genotype among β-thalassemia, Meanwhile, two α-mutation sites are found in this study which were not reported in the Database of Human Hemoglobin Variants and Thalassemias.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center