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Plant Mol Biol. 2019 Jun 15. doi: 10.1007/s11103-019-00892-0. [Epub ahead of print]

An integrated strategy to identify genes responsible for sesquiterpene biosynthesis in turmeric.

Author information

1
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
2
College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China.
3
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
4
School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China.
5
Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, China.
6
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. guojuan@wbgcas.cn.
7
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. huangluqi01@126.com.

Abstract

Metabolic module, gene expression pattern and PLS modeling were integrated to precisely identify the terpene synthase responsible for sesquiterpene formation. Functional characterization confirmed the feasibility and sensitivity of this strategy. Plant secondary metabolite biosynthetic pathway elucidation is crucial for the production of these compounds with metabolic engineering. In this study, an integrated strategy was employed to predict the gene function of sesquiterpene synthase (STS) genes using turmeric as a model. Parallel analysis of gene expression patterns and metabolite modules narrowed the candidates into an STS group in which the STSs showed a similar expression pattern. The projections to latent structures by means of partial least squares model was further employed to establish a clear relationship between the candidate STS genes and metabolites and to predict three STSs (ClTPS16, ClTPS15 and ClTPS14) involved in the biosynthesis of several sesquiterpene skeletons. Functional characterization revealed that zingiberene and β-sesquiphellandrene were the major products of ClTPS16, and β-eudesmol was produced by ClTPS15, both of which indicated the accuracy of the prediction. Functional characterization of a control STS, ClTPS1, produced a small amount of β-sesquiphellandrene, as predicted, which confirmed the sensitivity of metabolite module analysis. This integrated strategy provides a methodology for gene function predictions, which represents a substantial improvement in the elucidation of biosynthetic pathways in nonmodel plants.

KEYWORDS:

Functional prediction; Gene expression pattern; Metabolite module; PLS; Terpene synthase

PMID:
31203559
DOI:
10.1007/s11103-019-00892-0

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