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Plant Cell Physiol. 2019 Jun 14. pii: pcz117. doi: 10.1093/pcp/pcz117. [Epub ahead of print]

FAX2 mediates fatty acids export from plastid in developing Arabidopsis seeds.

Author information

1
Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
2
Institute of New Energy and Low-carbon Technology, Sichuan University, Chengdu, China.
3
Sichuan Natural Resource Institute, Chengdu, China.

Abstract

Vegetable oils are mainly stored in the form of triacylglycerol (TAG) in oilseeds. Fatty acids (FAs), one of the building blocks for TAG assembly, are synthesized in plastids and then exported to endoplasmic reticulum (ER) for storage oil synthesis. Recent study demonstrated that exportation of FAs from plastid was mediated by FAX (fatty acid export) family protein. However, the role of FAs exportation from plastid during seed oil accumulation has not been investigated. In this study, we found that FAX2 was highly expressed in developing Arabidopsis seeds and the expression level was consistent with FAs synthesis activity. FAX2 mutant seeds showed a ∼18% reduction of lipid levels compared with WT seeds. By contrast, overexpression of FAX2 enhanced seed lipid accumulation by up to 30%. The FAs export activity of FAX2 was confirmed by yeast mutant cell complement analysis. Our results showed that FAX2 could interact with other proteins to facilitate FAs transportation. Taken together, these results indicate that FAX2 mediated FAs export from plastid plays an important role for seed oil accumulation, and FAX2 can be used as a target gene for increasing lipid production in oilseeds.

KEYWORDS:

Arabidopsis; FAX2; lipid transport; seed oil content; triacylglycerol; vegetable oil

PMID:
31198959
DOI:
10.1093/pcp/pcz117

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