Format

Send to

Choose Destination
Science. 2019 Jun 14;364(6445):1095-1098. doi: 10.1126/science.aav8197.

Mutation of a bHLH transcription factor allowed almond domestication.

Author information

1
Department of Plant Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Spain. rsanchez@cebas.csic.es stefano.pavan@uniba.it.
2
Plant Biochemistry Laboratory, Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
3
VILLUM Research Center for Plant Plasticity, Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
4
Department of Soil, Plant and Food Science, University of Bari "Aldo Moro," 70126 Bari, Italy. rsanchez@cebas.csic.es stefano.pavan@uniba.it.
5
Institute of Biomedical Technologies, National Research Council (CNR), 70126 Bari, Italy.
6
Department of Soil, Plant and Food Science, University of Bari "Aldo Moro," 70126 Bari, Italy.
7
Sequentia Biotech SL, Campus de la UAB, 08193 Bellaterra, Barcelona, Spain.
8
Department of Plant Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Spain.
9
Arboriculture Research Group, Agroscope, Conthey, Switzerland.
10
Department of the Sciences of Agriculture, Food and Environment, University of Foggia, 71100 Foggia, Italy.
11
Transport Biology Section, Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.

Abstract

Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F1 population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase-encoding genes PdCYP79D16 and PdCYP71AN24, which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.

PMID:
31197015
DOI:
10.1126/science.aav8197

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center