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Anal Biochem. 2019 Sep 1;580:49-55. doi: 10.1016/j.ab.2019.06.005. Epub 2019 Jun 11.

An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent.

Author information

1
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao.
2
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, 999077, Hong Kong.
3
CAS Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. Electronic address: rjpei2011@sinano.ac.cn.
4
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, 999077, Hong Kong. Electronic address: edmondma@hkbu.edu.hk.
5
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao. Electronic address: duncanleung@um.edu.mo.

Abstract

A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.

KEYWORDS:

G-quadruplex; Herbal plant extracts; OTA; TRES

PMID:
31194944
DOI:
10.1016/j.ab.2019.06.005

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