A novel plasmid carrying carbapenem-resistant gene bla _KPC-2 in Pseudomonas aeruginosa

A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla _KPC-2 gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene bla _KPC-2. pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of bla _KPC-2 gene (ΔIS6-Tn3-ISKpn8-bla _KPC-2-ISKpn6-IS26).