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Cell Rep. 2019 Jun 11;27(11):3254-3268.e8. doi: 10.1016/j.celrep.2019.05.051.

Robust Control of a Brain-Persisting Parasite through MHC I Presentation by Infected Neurons.

Author information

1
Center for Pathophysiology Toulouse-Purpan (CPTP), INSERM, CNRS, University of Toulouse, 31024 Toulouse, France.
2
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
3
Center for Pathophysiology Toulouse-Purpan (CPTP), INSERM, CNRS, University of Toulouse, 31024 Toulouse, France. Electronic address: nicolas.blanchard@inserm.fr.

Abstract

Control of CNS pathogens by CD8 T cells is key to avoid fatal neuroinflammation. Yet, the modalities of MHC I presentation in the brain are poorly understood. Here, we analyze the antigen presentation mechanisms underlying CD8 T cell-mediated control of the Toxoplasma gondii parasite in the CNS. We show that MHC I presentation of an efficiently processed model antigen (GRA6-OVA), even when not expressed in the bradyzoite stage, reduces cyst burden and dampens encephalitis in C57BL/6 mice. Antigen presentation assays with infected primary neurons reveal a correlation between lower MHC I presentation of tachyzoite antigens by neurons and poor parasite control in vivo. Using conditional MHC I-deficient mice, we find that neuronal MHC I presentation is required for robust restriction of T. gondii in the CNS during chronic phase, showing the importance of MHC I presentation by CNS neurons in the control of a prevalent brain pathogen.

KEYWORDS:

CD8 T cell; Toxoplasma gondii; antigen presentation; brain infection; encephalitis; neuroinflammation; neuron; parasite

PMID:
31189109
DOI:
10.1016/j.celrep.2019.05.051
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