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Genet Mol Biol. 2019 Jun 10. pii: S1415-47572019005020103. doi: 10.1590/1678-4685-GMB-2018-0212. [Epub ahead of print]

[PROVISIONAL] The perturbed expression of m6A in parthenogenetic mouse embryos.

Author information

1
Laboratory Animal Center, College of Animal Science, Jilin University, Changchun 130062, China.
2
Department of Emergency, First Hospital, Jilin University, Changchun 130031, Jilin, China.

Abstract

Parthenogenetically activated oocytes cannot develop to term in mammals owing to abnormal epigenetic modifications. Methylation of the N6 position of adenosine (m6A) is a post-transcriptional epigenetic modification of RNA. To investigate the role of m6A methylation in parthenogenetic (PA) embryonic development, we analyzed METTL3, METTL14, FTO, ALKBH5, YTHDF2, IGF2BP1, and IGF2BP2 expression by quantitative real-time PCR. These genes were dynamically expressed during the 2-cell, 4-cell, 8-cell, and blastocyst stages of the embryo. Compared to normally fertilized embryos, the expression of these genes was perturbed in PA embryos, especially at the 8-cell stage. Furthermore, immunofluorescence was used to detect m6A expression. The results demonstrated that m6A expression decreased in the 2-cell stage, whereas it increased in the 8-cell stage of PA embryos. Taken together, these results suggest that the expression of RNA methylation-related genes was perturbed, leading to abnormal m6A modification during early development in PA embryos.

PMID:
31188932
DOI:
10.1590/1678-4685-GMB-2018-0212
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