Format

Send to

Choose Destination
Niger Postgrad Med J. 2019 Apr-Jun;26(2):80-86. doi: 10.4103/npmj.npmj_59_19.

Pattern of prevalent Hepatitis B virus genotypes in Zaria, Nigeria.

Author information

1
Department of Medicine, Ahmadu Bello University, Zaria, Nigeria.
2
Department of Medical Laboratory Science, Ahmadu Bello University, Zaria, Nigeria.
3
Research Unit, DNA LABS, Kaduna, Nigeria.
4
Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.
5
Department of Community Medicine, University of Jos, Jos, Nigeria.
6
Department of Medicine, Division of Infectious Diseases, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
7
Department of Obstetrics and Gynaecology, University of Jos, Jos, Nigeria.
8
Department of Medical Microbiology, Ahmadu Bello University, Zaria, Nigeria.

Abstract

Background:

Hepatitis B virus (HBV) is hyperendemic in Nigeria. Available literature reveal genotype E as being predominant in West Africa. This study aimed at identifying the current pattern and prevalent genotypes of HBV in Zaria, Nigeria.

Materials and Methods:

Four millilitre of blood was collected in ethylenediaminetetraacetic acid-container from each of 165 HBV surface antigen-positive participants recruited purposively from the gastroenterology clinic from May to August, 2017. Plasma was separated and frozen at -20°C till analysis. Multiplex-nested polymerase chain reaction using type-specific primers was used to identify the various HBV genotypes.

Results:

Median (and interquartile range) age of the participants was 31.0 (25.5-39.0) years, with males constituting 107 (64.8%). Majority (83.6%) of the samples analysed were HBV-DNA-positive with 82.6% of the HBV-DNA-positive samples being mixed genotype infections. Irrespective of mode of occurrence, five HBV genotypes were identified with HBV/E (97.1%) being the most predominant, followed by HBV/B (82.6%), HBV/A (24.6%), then HBV/C (17.4%), while HBV/D (0.7%) was the least prevalent.

Conclusion:

In most (99.1%) of the mixed-infection were a combination of genotype E, the predominant genotype, with other genotypes predominantly genotype B. HBV genotypes E, B, A, C and D are the prevalent genotypes in Zaria, Nigeria, as they occur in single genotype and in mixed-genotypes pattern.

KEYWORDS:

Genotypes; Nigeria; Zaria; hepatitis B Virus; mixed-infection

PMID:
31187746
PMCID:
PMC6626198
DOI:
10.4103/npmj.npmj_59_19
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
Loading ...
Support Center