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Small. 2019 Jun 11:e1901787. doi: 10.1002/smll.201901787. [Epub ahead of print]

Electrochemical Monitoring of Paclitaxel-Induced ROS Release from Mitochondria inside Single Cells.

Author information

1
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.

Abstract

Mitochondria are believed to be the major source of intracellular reactive oxygen species (ROS). However, in situ, real-time and quantitative monitoring of ROS release from mitochondria that are present in their cytosolic environment remains a great challenge. In this work, a platinized SiC@C nanowire electrode is placed into a single cell for in situ detection of ROS signals from intracellular mitochondria, and antineoplastic agent (paclitaxel) induced ROS production is successfully recorded. Further investigations indicate that complex IV (cytochrome c oxidase, COX) is the principal site for ROS generation, and significantly more ROS are generated from mitochondria in cancer cells than that from normal cells. This work provides an effective approach to directly monitor intracellular mitochondria by nanowire electrodes, and consequently obtains important physiological evidence on antineoplastic agent-induced ROS generation, which will be of great benefit for better understanding of chemotherapy at subcellular levels.

KEYWORDS:

amperometry; mitochondria; nanowire electrodes; paclitaxel; reactive oxygen species

PMID:
31183973
DOI:
10.1002/smll.201901787

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