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J Bacteriol. 2019 Jun 10. pii: JB.00314-19. doi: 10.1128/JB.00314-19. [Epub ahead of print]

YshB promotes intracellular replication and is required for Salmonella virulence.

Author information

1
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
2
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. zhoud@purdue.edu zhan2690@purdue.edu.

Abstract

Salmonella virulence requires the initial invasion of host cells followed by the modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5 kD protein named YshB that is involved in the intracellular survival of Salmonella We show here that yshB expression is upregulated upon entry into macrophages. When yshB expression is upregulated before bacterial entry, invasion efficiency is inhibited. Lack of YshB resulted in reduced bacterial survival within the macrophages and led to reduced virulence in a mouse model of infection.IMPORTANCE Salmonella gastroenteritis is one of the most common causes of food-borne disease, possibly affecting millions of people globally each year. Here we characterize the role of a novel small protein YshB in mediating Salmonella intracellular survival. This elucidation adds another layer to the body of knowledge of how this bacterium confers its intracellular survival.

PMID:
31182500
DOI:
10.1128/JB.00314-19

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