A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies

Comp Biochem Physiol Part D Genomics Proteomics. 2019 Sep:31:100599. doi: 10.1016/j.cbd.2019.100599. Epub 2019 May 31.

Abstract

Snake venoms are complex mixtures of a large number of distinct proteins and peptides with biological activity. Peptide spectral libraries are compilations of previously identified MS/MS spectra obtained from proteomics experiments. Here we present the generation and use of a Venom Peptidome and a Venom Proteome spectral library for the analysis of venom proteomes and peptidomes from distinct snake species.

Keywords: Database searching; Peptidome; Proteome; Snake venom; Spectral libraries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bothrops / metabolism
  • Crotalid Venoms / chemistry
  • Databases, Protein
  • Peptides / chemistry*
  • Proteome / chemistry
  • Proteomics / methods
  • Reptilian Proteins / chemistry*
  • Snake Venoms / chemistry*
  • Snakes / metabolism
  • Tandem Mass Spectrometry* / methods

Substances

  • Crotalid Venoms
  • Peptides
  • Proteome
  • Reptilian Proteins
  • Snake Venoms