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ACS Synth Biol. 2019 Jun 21;8(6):1257-1262. doi: 10.1021/acssynbio.9b00152. Epub 2019 Jun 13.

De Novo Production of the Plant-Derived Tropine and Pseudotropine in Yeast.

Ping Y1,2, Li X1,2, You W1,2, Li G1,2, Yang M1,2, Wei W1,2, Zhou Z1,2, Xiao Y1,2.

Author information

1
CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences , Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , 200032 , China.
2
University of Chinese Academy of Sciences , Beijing , 100039 , China.

Abstract

Tropine and pseudotropine with opposite stereospecific configurations as platform compounds are central building blocks in both biosynthesis and chemical synthesis of pharmacologically important tropane and nortropane alkaloids. The supply of plant-derived tropine and pseudotropine still heavily depends on either plant extraction or chemical synthesis. Advances in synthetic biology prompt the microbial synthesis of various valuable chemicals. With the biosynthetic pathway elucidation of tropine and pseudotropine in several Solanaceae plants, the key genes were sequentially identified. Here, the enzymes responsible for converting N-methylpyrrolinium into tropine and pseudotropine from Anisodus acutangulus were characterized. Reconstruction of the six-step biosynthetic pathways into Saccharomyces cerevisiae provides cell chassis producing tropine and pseudotropine with 0.13 and 0.08 mg/L titers from simple feedstocks in a shake flask, respectively. The strains described not only offer alternative sources of these central intermediates and their derived alkaloids but also provide platforms for pathway enzyme discovery.

KEYWORDS:

; pseudotropine; tropane alkaloid; tropine

PMID:
31181154
DOI:
10.1021/acssynbio.9b00152

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