Format

Send to

Choose Destination
Biologicals. 2019 Jul;60:36-41. doi: 10.1016/j.biologicals.2019.06.001. Epub 2019 Jun 7.

Evaluation of environment safety of a Japanese encephalitis live attenuated vaccine.

Author information

1
National Institutes for Food and Drug Control, Beijing 102629, PR China.
2
Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, PR China.
3
National Institutes for Food and Drug Control, Beijing 102629, PR China. Electronic address: wangjz@nifdc.org.cn.
4
National Institutes for Food and Drug Control, Beijing 102629, PR China. Electronic address: yuyongxin@nifdc.org.cn.
5
National Institutes for Food and Drug Control, Beijing 102629, PR China; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, PR China. Electronic address: liyuhua@nifdc.org.cn.

Abstract

JE vaccination is the most effective and economical method of preventing JE. A live attenuated JE vaccine has been widely used in many countries since 1989, playing an important role in controlling JE outbreaks. However, whether the large-scale use of the live attenuated JE vaccine will lead to the dissemination of the vaccine virus in the environment and whether reversion of the neuroattenuation of the virus will occur during the transmission process remain major concerns for some researchers. To evaluate the transmission of a live attenuated JEV vaccine in mosquitoes and hosts, JE SA14-14-2 attenuated vaccine virus was intrathoracically (i.t.) inoculated into Culex tritaeniorhynchus, a native vector. Subsequently, virus harvested from inoculated mosquitoes was inoculated into pigs, a mammalian reservoir. The virus was isolated from the pigs and passaged once again in Culex tritaeniorhynchus. The genome sequences and virulence of the passaged viruses were then investigated. While a few nucleotide substitutions occurred during passaging, there was no change in the encoded amino acids. After intracerebral (i.c.) inoculation of mice with the vaccine, no pathological effects were observed. In addition, virus virulence remained low after inoculation of suckling mouse brains. These results indicate that vaccination of individuals with the live vaccine will not result in transmission of the live SA14-14-2 vaccine virus through mosquito biting and virus amplified in pigs.

KEYWORDS:

Environment safety; Japanese encephalitis; Live attenuated vaccine; Transmission

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center