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Colloids Surf B Biointerfaces. 2019 Jun 3;181:489-497. doi: 10.1016/j.colsurfb.2019.06.003. [Epub ahead of print]

Electrochemical and optical investigation of dental pulp stem cell adhesion on modified porous silicon scaffolds.

Author information

1
Université de Monastir, Faculté de Médecine de Monastir, Laboratoire des Interfaces et Matériaux Avancés, LR11ES55, 5000, Monastir, Tunisia. Electronic address: inessoussi88@gmail.com.
2
Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Laboratoire Matériaux, Traitement et Analyse (LMTA), BiotechPole, Sidi-Thabet, 2032, Ariana, Tunisia.
3
LBN, Montpellier University, Montpellier, France.
4
NANOMISENE Lab, LR16CRMN01, Centre for Research on Microelectronics and Nanotechnology CRMN of Sousse, Technopark of Sousse, B.P. 334, Sahloul, 4034, Sousse, Tunisia.
5
Laboratoire Charles Coulomb (L2C), Université de Montpellier, CNRS, Montpellier, France.
6
Laboratoire de Micro-Optoélectronique et Nanostructures, Faculté des Sciences de Monastir, Université de Monastir, LR99ES29, 5000, Monastir, Tunisia.
7
Institut Charles Gerhardt Montpellier (ICGM), UMR 5253, Université de Montpellier 2, Place Eugène Bataillon, 34095, Montpellier Cedex 05, France.
8
Université de Monastir, Faculté de Médecine de Monastir, Laboratoire des Interfaces et Matériaux Avancés, LR11ES55, 5000, Monastir, Tunisia.

Abstract

Extensive use of porous silicon (PSi) for tissue engineering is due to its convenient properties as it is both nontoxic and bioresorbable. Moreover, PSi surface modification is an important step to enhance cell adhesion and proliferation. In this work, a combination of optical and electrochemical studies is performed to elaborate a suitable PSi multilayer substrate for cell culture. For this study, we modified PSi surface by silanization and antibody grafting (APTES-anti STRO1), the 12-mer specific peptide to silicon p + type coating and the peptide modified with the antibody recognition sequence. Electrochemical characterization of PSi multilayers is performed to investigate its electrical behavior, determine the optimal measuring conditions and reveal the most stable PSi surfaces. Then, the behavior of dental pulp stem cells (DPSC) was investigated on various modified PSi surfaces. An electrochemical method was applied for the first time monitoring the electrical behavior of stem cell adhesion. The cells electrochemical behavior depends on the nature of the surface coating and the peptide-anti STRO1 improved adhesion and cell spreading onto the PSi surface compared to bare surface and the one coated with the peptide. Fluorescent microscopy revealed that all surface modification methods enhance cell adhesion compared to the bare PSi surface. An increased cell number is observed on APTES-anti STRO1, peptide and peptide-anti STRO1 coated PSi. The peptide-anti STRO1 provided the best cell proliferation results suggesting the improved accessibility of the recognition fragment of the antibody anti-STRO1.

KEYWORDS:

Dental pulp stem cell adhesion; Impedance measurement; Mesoporous silicon; Specific peptide; Surface modification

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