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EMBO J. 2019 Jun 7. pii: e100640. doi: 10.15252/embj.2018100640. [Epub ahead of print]

A specialised SKI complex assists the cytoplasmic RNA exosome in the absence of direct association with ribosomes.

Author information

1
Génétique des Interactions Macromoléculaires, Institut Pasteur, UMR3525 CNRS, Paris, France.
2
Sorbonne Université, Collège Doctoral, Paris, France.
3
Hub Bioinformatique et Biostatistique, Institut Pasteur - C3BI, USR 3756 IP CNRS, Paris, France.
4
Department of Medicine, McGill University Health Centre, Montréal, QC, Canada.
5
Génétique des Interactions Macromoléculaires, Institut Pasteur, UMR3525 CNRS, Paris, France jacquier@pasteur.fr mfromont@pasteur.fr.

Abstract

The Ski2-Ski3-Ski8 (SKI) complex assists the RNA exosome during the 3' to 5' degradation of cytoplasmic transcripts. Previous reports showed that the SKI complex is involved in the 3' to 5' degradation of mRNAs, including 3' untranslated regions (UTRs) and devoid of ribosomes. Paradoxically, we recently showed that the SKI complex directly interacts with ribosomes during the co-translational mRNA decay and that this interaction is necessary for its RNA degradation promoting activity. Here, we characterised a new SKI-associated factor, Ska1, that associates with a subpopulation of the SKI complex. We showed that Ska1 is specifically involved in the degradation of long 3'UTR-containing mRNAs, poorly translated mRNAs as well as other RNA regions not associated with ribosomes, such as cytoplasmic lncRNAs. We further show that the overexpression of SKA1 antagonises the SKI-ribosome association. We propose that the Ska1-SKI complex assists the cytoplasmic exosome in the absence of direct association of the SKI complex with ribosomes.

KEYWORDS:

Saccharomyces cerevisiae ; SKI complex; exosome; mRNA decay pathway; ribosome

PMID:
31175107
DOI:
10.15252/embj.2018100640

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