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Int Immunopharmacol. 2019 Aug;73:502-514. doi: 10.1016/j.intimp.2019.05.045. Epub 2019 Jun 4.

Immunopathological effects of Agaricus blazei Murill polysaccharides against Schistosoma mansoni infection by Th1 and NK1 cells differentiation.

Author information

1
Department of Microbiology and Immunology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan; M.Sc. Program in Tropical Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan.
2
Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan.
3
Department of Dermatology, National Taiwan University Hospital Hsinchu Branch, Hsinchu, Taiwan.
4
Department of Biochemistry, College of Medicine, Tzu Chi University, Hualien, Taiwan. Electronic address: pengsy@mail.tcu.edu.tw.
5
Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan.
6
Biotechnology Center, Grape King Inc., Chungli, Taiwan.
7
Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Center for International Tropical Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
8
Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Center for International Tropical Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: bonjovi@tmu.edu.tw.

Abstract

In this study, we examined the ability of A. blazei Murill polysaccharides (AB-PS) to activate the immune system in vivo and the protective activity exhibited against parasitic S. mansoni in the murine model. AB-PS treatment significantly reduced the worm and egg burden in infected BALB/c and C57BL/6 mice with dose- and time-dependent manners. Additionally, a dose- and time-dependent expression of IL-2, INF-γ, and TNF-α cytokines was also observed in both strains of mice treatments. Using T1/T2 doubly transgenic mice, we demonstrated that AB-PS-treated mice splenocytes initiated early differentiation of Th1 and NK1 cells, which was consistent with the reduction course of Schistosoma infection. Although AB-PS treatment enhanced the Th1 response, it did not suppress Th2 cell activity in treated mice. Histopathological data of the livers showed AB-PS treatment significantly attenuated the liver fibrosis induced by S. mansoni eggs. AB-PS augmented type-1 responses by inducing Th1 and NK1 cell differentiation to effectively decrease the infection rate of S. mansoni. Furthermore, AB-PS treatment may not only inhibit the schistosome infection, but also improving the pathological effects of granulomas formation. This study provides evidence for a novel therapeutic potential, by which A. blazei Murill may be used to treat or prevent schistosome infection.

KEYWORDS:

Agaricus blazei Murill polysaccharides; Immunomodulation; NK cell; Schistosoma mansoni; T1/T2 doubly transgenic mice

PMID:
31173972
DOI:
10.1016/j.intimp.2019.05.045
[Indexed for MEDLINE]

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