Format

Send to

Choose Destination
Cell Stem Cell. 2019 Jun 6;24(6):860-876. doi: 10.1016/j.stem.2019.05.005.

Engineering Stem Cell Self-organization to Build Better Organoids.

Author information

1
Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
2
Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Institute of Chemical Sciences and Engineering, School of Basic Science (SB), EPFL, Lausanne, Switzerland. Electronic address: matthias.lutolf@epfl.ch.

Abstract

Organoids form through self-organization processes in which initially homogeneous populations of stem cells spontaneously break symmetry and undergo in-vivo-like pattern formation and morphogenesis, though the processes controlling this are poorly characterized. While these in vitro self-organized tissues far exceed the microscopic and functional complexity obtained by current tissue engineering technologies, they are non-physiological in shape and size and have limited function and lifespan. Here, we discuss how engineering efforts for guiding stem-cell-based development at multiple stages can form the basis for the assembly of highly complex and rationally designed self-organizing multicellular systems with increased robustness and physiological relevance.

KEYWORDS:

biofabrication; biomaterial; extracellular matrix; morphogenesis; organoid; patterning; self-organization; stem cell; symmetry breaking; tissue engineering

PMID:
31173716
DOI:
10.1016/j.stem.2019.05.005

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center