Format

Send to

Choose Destination
Semin Ophthalmol. 2019 Jun 6:1-9. doi: 10.1080/08820538.2019.1620800. [Epub ahead of print]

A Review of Next-Generation Sequencing (NGS): Applications to the Diagnosis of Ocular Infectious Diseases.

Author information

1
a David G Cogan Laboratory of Ophthalmic Pathology, Massachusetts Eye and Ear Infirmary , Harvard Medical School , Boston , MA , USA.

Abstract

Purpose: To review the value of next-generation sequencing (NGS) in identifying the pathogens which cause ocular infections, thereby facilitating prompt initiation of treatment with an optimal anti-microbial regimen. Both contemporary and futuristic approaches to identifying pathogens in ocular infections are covered in this brief overview. Methods: Review of the peer reviewed literature on conventional and advanced methods as applied to the diagnosis of infectious diseases of the eye. Conclusion: NGS is a novel technology for identifying the pathogens responsible for ocular infections with the potential to improve the accuracy and speed of diagnosis and hastening the selection of the best therapy.

KEYWORDS:

Next-generation sequencing (NGS); conjunctivitis; dacryocystitis; endophthalmitis; keratitis; microbial whole-genome sequencing (MWGS); ocular infection; review article; targeted Amplicon sequencing (TAS); uveitis

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center