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Semin Ophthalmol. 2019;34(4):223-231. doi: 10.1080/08820538.2019.1620800. Epub 2019 Jun 6.

A Review of Next-Generation Sequencing (NGS): Applications to the Diagnosis of Ocular Infectious Diseases.

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a David G Cogan Laboratory of Ophthalmic Pathology, Massachusetts Eye and Ear Infirmary , Harvard Medical School , Boston , MA , USA.


Purpose: To review the value of next-generation sequencing (NGS) in identifying the pathogens which cause ocular infections, thereby facilitating prompt initiation of treatment with an optimal anti-microbial regimen. Both contemporary and futuristic approaches to identifying pathogens in ocular infections are covered in this brief overview. Methods: Review of the peer reviewed literature on conventional and advanced methods as applied to the diagnosis of infectious diseases of the eye. Conclusion: NGS is a novel technology for identifying the pathogens responsible for ocular infections with the potential to improve the accuracy and speed of diagnosis and hastening the selection of the best therapy.


Next-generation sequencing (NGS); conjunctivitis; dacryocystitis; endophthalmitis; keratitis; microbial whole-genome sequencing (MWGS); ocular infection; review article; targeted Amplicon sequencing (TAS); uveitis

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