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J Infect Dis. 2019 Aug 30;220(7):1209-1218. doi: 10.1093/infdis/jiz274.

Repertoire and Neutralizing Activity of Antibodies Against Hepatitis C Virus E2 Peptide in Patients With Spontaneous Resolution of Hepatitis C.

Author information

1
Laboratory for Clinical and Experimental Hepatology, Section of Hepatology, Clinic for Gastroenterology and Rheumatology, University Clinic Leipzig, Leipzig, Germany.
2
Division of B Cell Immunology, German Cancer Research Center, Heidelberg, Germany.
3
Max von Pettenkofer Institute, Munich, Germany.
4
Inserm U1110, University of Strasbourg, France.
5
Division of Infection and Immunity, University College London, United Kingdom.
6
Clinic for Hepatology and Gastroenterology, Charité, CVK, Berlin, Germany.

Abstract

Neutralizing antibodies can prevent hepatitis C virus (HCV) infection, one of the leading causes of cirrhosis and liver cancer. Here, we characterized the immunoglobulin repertoire of memory B-cell antibodies against a linear epitope in the central front layer of the HCV envelope (E2; amino acids 483-499) in patients who were infected in a single-source outbreak. A reverse transcription polymerase chain reaction-based immunoglobulin gene cloning and recombinant expression approach was used to express monoclonal antibodies from HCV E2 peptide-binding immunoglobulin G-positive memory B cells. We identified highly mutated antibodies with a neutralizing effect in vitro against different genotype isolates sharing similar gene features. Our data confirm the importance of VH1-69 use for neutralizing activity. The data offer a promising basis for vaccine research and the use of anti-E2 antibodies as a means of passive immunization.

KEYWORDS:

HCV; HCV envelope (E2) protein; anti-D cohort; memory B cells; neutralizing antibodies

PMID:
31165162
DOI:
10.1093/infdis/jiz274

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