Format

Send to

Choose Destination
Nat Commun. 2019 Jun 3;10(1):2395. doi: 10.1038/s41467-019-10291-0.

A comprehensive single cell transcriptional landscape of human hematopoietic progenitors.

Author information

1
Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA, 02115, USA.
2
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
3
Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
4
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA. Allon_Klein@hms.harvard.edu.
5
Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA, 02115, USA. l.biasco@ucl.ac.uk.
6
University College of London (UCL), Great Ormond Street Institute of Child Health Faculty of Population Health Sciences, London, WC1N 1EH, UK. l.biasco@ucl.ac.uk.

Abstract

Hematopoietic Stem/Progenitor cells (HSPCs) are endowed with the role of maintaining a diverse pool of blood cells throughout the human life. Despite recent efforts, the nature of the early cell fate decisions remains contentious. Using single-cell RNA-Seq, we show that existing approaches to stratify bone marrow CD34+ cells reveal a hierarchically-structured transcriptional landscape of hematopoietic differentiation. Still, this landscape misses important early fate decisions. We here provide a broader transcriptional profiling of bone marrow lineage negative hematopoietic progenitors that recovers a key missing branchpoint into basophils and expands our understanding of the underlying structure of early adult human haematopoiesis. We also show that this map has strong similarities in topology and gene expression to that found in mouse. Finally, we identify the sialomucin CD164, as a reliable marker for the earliest branches of HSPCs specification and we showed how its use can foster the design of alternative transplantation cell products.

PMID:
31160568
PMCID:
PMC6546699
DOI:
10.1038/s41467-019-10291-0
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center