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BMC Plant Biol. 2019 Jun 3;19(1):234. doi: 10.1186/s12870-019-1810-2.

Overexpression of a soybean YABBY gene, GmFILa, causes leaf curling in Arabidopsis thaliana.

Yang H1,2, Shi G3, Li X1, Hu D1, Cui Y1, Hou J1, Yu D1,2, Huang F4.

Author information

1
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.
2
School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
3
Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
4
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China. fhuang@njau.edu.cn.

Abstract

BACKGROUND:

YABBY genes play important roles in the growth and polar establishment of lateral organs such as leaves and floral organs in angiosperms. However, the functions of YABBY homologous genes are largely unknown in soybean.

RESULTS:

In this study, we identified GmFILa encoding a YABBY transcription factor belonging to FIL subfamily. In situ mRNA hybridization analysis indicated that GmFILa had specific expression patterns in leaf as well as in flower bud primordia. Ectopic expression of GmFILa in Arabidopsis thaliana altered the partial abaxialization of the adaxial epidermises of leaves. Besides, GmFILa transgenic plants also exhibited longer flowering period and inhibition of shoot apical meristem (SAM) development compared to the wild type plants. Digital expression data and quantitative real-time polymerase chain reaction (qRT-PCR) analysis demonstrated that the expression of GmFILa was induced by biotic and abiotic stresses and hormone treatments. Transcriptome analysis suggested that overexpressing GmFILa yielded 82 significant differentially expressed genes (DEGs) in Arabidopsis leaves, which can be classified into transcription factors, transporters, and genes involved in growth and development, metabolism, signal transduction, redox reaction and stress response.

CONCLUSIONS:

These results not only demonstrate the roles of GmFILa involved in leaf adaxial-abaxial polarity in Arabidopsis, but also help to reveal the molecular regulatory mechanism of GmFILa based on the transcriptomic data.

KEYWORDS:

Adaxial-abaxial polarity; Arabidopsis; GmFILa; Leaf; Soybean; YABBY

PMID:
31159746
PMCID:
PMC6547562
DOI:
10.1186/s12870-019-1810-2
[Indexed for MEDLINE]
Free PMC Article

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