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Spectrochim Acta A Mol Biomol Spectrosc. 2019 Oct 5;221:117187. doi: 10.1016/j.saa.2019.117187. Epub 2019 May 28.

Modulation of fluorescence sensing properties of coumarin-based fluorescent probe for H2S and its application in cell imaging.

Author information

1
School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.
2
Key Laboratory of Coal Science and Technology of Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China. Electronic address: baihui@tyut.edu.cn.
3
School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China. Electronic address: wangqm@yctu.edu.cn.

Abstract

A coumarin-based turn on probe, named 3-((E)-3-(1H-indol-3-yl)acryloyl)-2H-chromen-2-one(IAC) was designed and synthesized for the detection of H2S in aqueous medium. IAC showed no obvious red fluorescence in Tris-HCl(pH = 7.2, 60% DMF), a 28.2-fold fluorescence enhancement was found when 40 equiv. H2S was added. Other analytes such as anions, metal ions and GSH, Cys, Gly did no significant fluorescence enhancement at 580 nm to IAC. The red fluorescence enhancement mechanism between IAC and H2S was considered by DFT and ESI-MS. Overall, IAC was successfully applied in cell imaging.

KEYWORDS:

Cell imaging; Coumarin-based; Fluorescent probe; H(2)S; Mechanism

PMID:
31158759
DOI:
10.1016/j.saa.2019.117187

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