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J Mol Biol. 2019 May 31. pii: S0022-2836(19)30323-7. doi: 10.1016/j.jmb.2019.05.039. [Epub ahead of print]

Tol Energy-Driven Localization of Pal and Anchoring to the Peptidoglycan Promote Outer-Membrane Constriction.

Author information

1
Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ-CNRS, Marseille, France.
2
Laboratoire de Chimie Bactérienne, UMR7283, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ-CNRS, Marseille, France.
3
Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ-CNRS, Marseille, France. Electronic address: duche@imm.cnrs.fr.

Abstract

During cell division, gram-negative bacteria must coordinate inner-membrane invagination, peptidoglycan synthesis and cleavage and outer-membrane (OM) constriction. The OM constriction remains largely enigmatic, and the nature of this process, passive or active, is under debate. The proton-motive force-dependent Tol-Pal system performs a network of interactions within these three compartments. Here we confirm that the trans-envelope Tol-Pal complex accumulates at constriction site in Escherichia coli. We show that the inner-membrane complex composed of TolA, TolQ and TolR recruits the OM complex TolB-Pal to the septum, in an energy-dependent process. Pal recruitment then allows its binding to peptidoglycan and subsequently OM constriction. Our results provide evidence that the constriction of the OM is an energized process.

KEYWORDS:

OM invagination; Tol–Pal system; membrane vesicles; molecular motor; septum

PMID:
31153904
DOI:
10.1016/j.jmb.2019.05.039

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