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Nutr Metab Cardiovasc Dis. 2019 May 4. pii: S0939-4753(19)30121-8. doi: 10.1016/j.numecd.2019.04.005. [Epub ahead of print]

Soy isoflavones (from Glycine max) preserves hepatocellular free radical defense system potential and ameliorates inflammatory response in high fat fed ovariectomised Wistar rats: The molecular mechanisms.

Author information

1
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry, 605 006, India.
2
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry, 605 006, India. Electronic address: sankarjipmer@gmail.com.

Abstract

BACKGROUND AND AIM:

Post menopausal women are more prone to develop metabolic syndrome (MetS) components; insulin resistance, type2 diabetes, dyslipidemia, atherosclerosis and obesity than premenopausal women. Oxidative stress (OxS) and inflammation (INF) are emerged as major triggers for these metabolic consequences. However, the pathogenesis of OxS and inflammation after menopause is only partially understood which is yet to be elucidated. The lack of safe/effective conventional treatments for postmenopausal women has changed the focus to natural products as the alternative remedy. We investigated the protective effects of soy isoflavones (SIF) on the pathogenesis of OxS and INF in an experimental model of postmenopausal obesity.

METHODS AND RESULTS:

Soy isoflavones were extracted and analyzed by HPLC method. In animal experiments, the sham operated and ovariectomised (OVX) Wistar rats were fed with either normal chow or high fat diet (HFD) in the presence and absence of soy isoflavones (150 mg/kg bw/day/rat) for eight weeks. The blood and tissue OxS and INF signaling parameters were studied using appropriate biochemical and molecular techniques. Proteomics studies revealed that, OVX and HFD per se caused OxS and INF. This was attributed by suppressed expression of Nrf2, HO-1 and catalase (CAT) in association with enhanced expression of MPO and inflammatory proteins; pNF-kBp65(Ser536), IKKβ, COX-2,PKCα, TNFα, IL-6. All these metabolic derangements were further augmented when OVX was followed by HFD, suggesting the synergistic role of postmenopausal state and intake of fat rich food on the development of oxidative stress and inflammation.

CONCLUSIONS:

Soy isoflavones significantly alleviated these metabolic complications, suggesting the use of this natural phytoestrogen as an alternative strategy/remedy for relieving hepatic oxidative stress and inflammation associated with postmenopausal women.

KEYWORDS:

Free radical defense system; Inflammatory response; Oxidative stress; Postmenopausal obesity; Soy isoflavones

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