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Carbohydr Polym. 2019 Sep 1;219:405-413. doi: 10.1016/j.carbpol.2019.05.028. Epub 2019 May 9.

N-alkylated chitosan/graphene oxide porous sponge for rapid and effective hemostasis in emergency situations.

Author information

1
Department of Polymer Science and Technology, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Institute of Medical Support Technology, Academy of Military Sciences, Tianjin, 300161, China.
2
Research Center of Military Standard, Academy of Military Sciences, Beijing, 100141, China; Institute of Medical Support Technology, Academy of Military Sciences, Tianjin, 300161, China. Electronic address: janeamms@aliyun.com.
3
Institute of Medical Support Technology, Academy of Military Sciences, Tianjin, 300161, China.
4
Department of Polymer Science and Technology, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
5
Department of Polymer Science and Technology, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
6
Department of Polymer Science and Technology, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. Electronic address: dengliandong@tju.edu.cn.

Abstract

N-alkylated chitosan (AC) sponges and graphene oxide (GO) sponges are promising candidates for emergency hemostat. However, AC sponges have weak mechanical strength and GO sponges may induce toxicity. To overcome these problems, a series of AC/GO composite spongs (ACGS) were prepared with various ratios (GO/AC, 0%, 5%, 10%, and 20%) using a dilute solution freeze phase separation and drying process. The sponges exhibit excellent absorption capacity, mechanical stability, and biocompatibility. In serial in vitro clotting tests, the higher the ratio of GO, the better the coagulation efficiency. ACGS with 20% ratio of GO (ACGS20) has shorter hemostatic time than Celox in a rabbit femoral injury test. Moreover, ACGS20 can accelerate erythrocyte and platelet adhesion. CD62p and intracellular Ca2+ measurements show that ACGS20 can promote the release of intracellular Ca2+ and stimulate platelet activation. These results suggest that ACGS20 is a good candidate composition for a safe and efficacious hemostatic dressing.

KEYWORDS:

Graphene oxide; Hemostasis; Hemostatic sponge; N-alkylated chitosan; Platelet

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