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Int J Mol Sci. 2019 May 30;20(11). pii: E2661. doi: 10.3390/ijms20112661.

P3HT:Bebq2-Based Photovoltaic Device Enhances Differentiation of hiPSC-Derived Retinal Ganglion Cells.

Hsu CC1,2, Lin YY3, Yang TC4, Yarmishyn AA5, Lin TW6, Chang YL7,8,9, Hwang DK10,11, Wang CY12,13,14, Liu YY15,16,17, Lo WL18,19,20, Peng CH21, Chen SJ22,23, Yang YP24,25,26.

Author information

1
Department of Ophthalmology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. chihchienym@gmail.com.
2
Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan. chihchienym@gmail.com.
3
Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan. s19609005@gm.ym.edu.tw.
4
Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, Taiwan. a8434040@hotmail.com.
5
Department of Ophthalmology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. yarmishyn@gmail.com.
6
Department of Ophthalmology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. backyard0826@gmail.com.
7
Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan. ylchang@vghtpe.gov.tw.
8
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan. ylchang@vghtpe.gov.tw.
9
Department of Pharmacology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. ylchang@vghtpe.gov.tw.
10
Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 40705, Taiwan. m95gbk@hotmail.com.
11
Department of Public Health and Institute of Public Health, National Yang-Ming University, Taipei 11217, Taiwan. m95gbk@hotmail.com.
12
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan. wangcy@vghtpe.gov.tw.
13
Department of Critical Care Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan. wangcy@vghtpe.gov.tw.
14
Division of Trauma, Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan. wangcy@vghtpe.gov.tw.
15
Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan. yyliu@vghtpe.gov.tw.
16
Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, Taiwan. yyliu@vghtpe.gov.tw.
17
Department of Chest, Taipei Veterans General Hospital, Taipei 11217, Taiwan. yyliu@vghtpe.gov.tw.
18
Institute of Oral Biology, National Yang-Ming University, Taipei 11211, Taiwan. wllo@vghtpe.gov.tw.
19
Division of Oral and Maxillofacial Surgery, Department of Stomatology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. wllo@vghtpe.gov.tw.
20
Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei 11211, Taiwan. wllo@vghtpe.gov.tw.
21
Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital & Fu-Jen Catholic University, Taipei 11101, Taiwan. chpeng1008@gmail.com.
22
Department of Ophthalmology, Taipei Veterans General Hospital, Taipei 11217, Taiwan. sjchen96@gmail.com.
23
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan. sjchen96@gmail.com.
24
Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, Taiwan. molly0103@gmail.com.
25
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan. molly0103@gmail.com.
26
School of Pharmaceutical Sciences, National Yang-Ming University, Taipei 11211, Taiwan. molly0103@gmail.com.

Abstract

Electric field stimulation is known to affect various cellular processes, including cell fate specification and differentiation, particularly towards neuronal lineages. This makes it a promising therapeutic strategy to stimulate regeneration of neuronal tissues. Retinal ganglion cells (RGCs) is a type of neural cells of the retina responsible for transduction of visual signals from the retina to the brain cortex, and is often degenerated in various blindness-causing retinal diseases. The organic photovoltaic materials such as poly-3-hexylthiophene (P3HT) can generate electric current upon illumination with light of the visible spectrum, and possesses several advantageous properties, including light weight, flexibility and high biocompatibility, which makes them a highly promising tool for electric stimulation of cells in vitro and in vivo. In this study, we tested the ability to generate photocurrent by several formulations of blend (bulk heterojunction) of P3HT (which is electron donor material) with several electron acceptor materials, including Alq3 and bis(10-hydroxybenzo[h]quinolinato)beryllium (Bebq2). We found that the photovoltaic device based on bulk heterojunction of P3HT with Bebq2 could generate photocurrent when illuminated by both green laser and visible spectrum light. We tested the growth and differentiation capacity of human induced pluripotent stem cells (hiPSC)-derived RGCs when grown in interface with such photostimulated device, and found that they were significantly increased. The application of P3HT:Bebq2-formulation of photovoltaic device has a great potential for developments in retinal transplantation, nerve repair and tissue engineering approaches of treatment of retinal degeneration.

KEYWORDS:

induced pluripotent stem cells (iPSC); photovoltaic; poly-3-hexylthiophene (P3HT); retinal ganglia cells (RGC)

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