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Theranostics. 2019 Apr 12;9(8):2325-2345. doi: 10.7150/thno.30030. eCollection 2019.

Biodistribution of gadolinium- and near infrared-labeled human umbilical cord mesenchymal stromal cell-derived exosomes in tumor bearing mice.

Author information

1
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
2
Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA.
3
Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
4
Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State University, Manhattan, KS 66506, USA.
5
Midwest Institute of Comparative Stem Cell Biology, Kansas State University, Manhattan, KS 66506, USA.

Abstract

We speculate that exosomes derived from human umbilical cord mesenchymal stromal cells (HUC-MSCs) will accumulate within tumors and have the potential for both tumor location or drug delivery. Methods: To determine proof of concept, HUC-MSC exosomes were labeled with an MRI contrast agent, gadolinium, or a near infrared dye. Exosome accumulation within ectopic osteosarcoma tumor-bearing mice was determined by 14.1 T MRI or bioimaging over 24-48 h after injection. In vitro studies examine the accumulation and physiological effect of exosomes on human and mouse osteosarcoma cell lines by MTT assay, confocal microscopy, and flow cytometry. Results: Systemic HUC-MSC exosomes accumulated continuously in tumor over a 24-48 h post-injection period. In contrast, synthetic lipid nanoparticles accumulate in tumor only for the first 3 h post-injection. Conclusion: These results suggest that HUC-MSCs exosomes accumulate within human or mouse osteosarcoma cells in vitro and in vivo over a 24 to 48 h after infusion.

KEYWORDS:

Extracellular vesicles; ectopic osteosarcoma model; gadolinium nanoparticle.; non-invasive imaging

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

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