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Methods Mol Biol. 2019;1988:375-386. doi: 10.1007/978-1-4939-9450-2_26.

Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies.

Author information

1
Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
2
Benaroya Research Institute at Virginia Mason, Seattle, WA, USA. bkwok@benaroyaresearch.org.
3
Department of Medicine, University of Washington, Seattle, WA, USA. bkwok@benaroyaresearch.org.

Abstract

The development of peptide-MHC class II multimers has provided a novel approach in studying antigen-specific CD4+ T-cells and extended the knowledge of these T cells in various disease settings, including infectious diseases, autoimmune diseases, cancer, and allergies. This chapter discusses the various applications of the peptide-MHC class II multimer technologies, specifically their uses in the evaluation of antigen-specific CD4+ T-cells ex vivo, and their uses in epitope identification.

KEYWORDS:

Epitopes; Peptide–MHC class II multimers; Peptide–MHC class II tetramers; T-cell receptor; T-cells

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