Format

Send to

Choose Destination
Sci Rep. 2019 May 30;9(1):8087. doi: 10.1038/s41598-019-44298-w.

A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips.

Author information

1
Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, China.
2
Endodontics, Taian Stomatology Hospital, 261-1 Lingshan Avenue, Taian, 271000, China.
3
School of Microelectronics, Shandong University, 27 South Shanda Road, Jinan, 250010, China.
4
State Key Laboratory of Crystal Materials, Shandong University, 27 South Shanda Road, Jinan, 250010, China.
5
Institute for Advanced Interdisciplinary Research, Jinan University, Jinan, 250022, China.
6
Cheeloo Health Science Center, Shandong University, 44 West Wenhua Road, Jinan, 250012, China. qqg@sdu.edu.cn.
7
Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, China. hanlin@sdu.edu.cn.

Abstract

Due to the antibacterial resistance crisis, developing new antibacterials is of particular interest. In this study, we combined the antifungal drug amphotericin B with 50,520 different small molecule compounds obtained from the Chinese National Compound Library in an attempt to improve its efficacy against Candida albicans persister cells. To systematically study the antifungal effect of each compound, we utilized custom-designed high-throughput microfluidic chips. Our microfluidic chips contained microchannels ranging from 3 µm to 5 µm in width to allow Candida albicans cells to line up one-by-one to facilitate fluorescence-microscope viewing. After screening, we were left with 10 small molecule compounds that improved the antifungal effects of amphotericin B more than 30% against Candida albicans persister cells.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center