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Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12494-12499. doi: 10.1073/pnas.1901067116. Epub 2019 May 30.

ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain.

Author information

1
Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, Korea.
2
College of Pharmacy, Chung-ang University, 06974 Seoul, Korea.
3
Division of Bio-Medical Science and Technology, Korea Institute of Science and Technology, 02792 Seoul, Korea.
4
Department of Biomedical Engineering, Hanyang University, 04763 Seoul, Korea.
5
Department of Anatomy, College of Medicine, Catholic University, 06591 Seoul, Korea.
6
Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, Korea; utoh@kist.re.kr.

Abstract

Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.

KEYWORDS:

Anoctamin 1; TMEM16A; cortical development; neural stem cell; radial glial cell

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