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Genes (Basel). 2019 May 29;10(6). pii: E415. doi: 10.3390/genes10060415.

A (3D-Nuclear) Space Odyssey: Making Sense of Hi-C Maps.

Author information

1
Epigenetics and Sex Development Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin 10115, Germany. Irene.MotaGomez-Argente@mdc-berlin.de.
2
Epigenetics and Sex Development Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin 10115, Germany. Dario.Lupianez@mdc-berlin.de.

Abstract

Three-dimensional (3D)-chromatin organization is critical for proper enhancer-promoter communication and, therefore, for a precise execution of the transcriptional programs governing cellular processes. The emergence of Chromosome Conformation Capture (3C) methods, in particular Hi-C, has allowed the investigation of chromatin interactions on a genome-wide scale, revealing the existence of overlapping molecular mechanisms that we are just starting to decipher. Therefore, disentangling Hi-C signal into these individual components is essential to provide meaningful biological data interpretation. Here, we discuss emerging views on the molecular forces shaping the genome in 3D, with a focus on their respective contributions and interdependence. We discuss Hi-C data at both population and single-cell levels, thus providing criteria to interpret genomic function in the 3D-nuclear space.

KEYWORDS:

3D-chromatin organization; chromosome conformation capture; long-range gene regulation

PMID:
31146487
DOI:
10.3390/genes10060415
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