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Plant Methods. 2019 May 23;15:53. doi: 10.1186/s13007-019-0439-3. eCollection 2019.

Enhancement of tanshinone production in Salvia miltiorrhiza hairy root cultures by metabolic engineering.

Wei T#1,2,3, Gao Y#2, Deng K#3,4, Zhang L2, Yang M2, Liu X3,4, Qi C3,4, Wang C2, Song W2, Zhang Y3,4, Chen C2.

Author information

1
1National Pesticide Engineering Research Center (Tianjin), Nankai University, Tianjin, 300071 People's Republic of China.
2
2College of Life Sciences, Nankai University, Tianjin, 300071 People's Republic of China.
3
3School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 People's Republic of China.
4
4Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054 People's Republic of China.
#
Contributed equally

Abstract

Background:

Tanshinones are diterpenoid compounds that are used to treat cardiovascular diseases. As current extraction methods for tanshinones are inefficient, there is a pressing need to improve the production of these bioactive compounds to meet increasing demand.

Results:

Overexpression of SmMDS (2-c-methyl-d-erythritol 2,4-cyclodiphosphate synthase, a tanshinone biosynthesis gene) in transgenic Salvia miltiorrhiza hairy roots significantly increased the tanshinone yield compared to the control, and total tanshinone content in SmMDS-overexpressing lines increased after elicitor treatment. Total tanshinones increased to 2.5, 2.3, and 3.2 mg/g DW (dry weight) following treatment with Ag+, YE (yeast extract), and MJ (methyl jasmonate), respectively, compared with the non-induced transgenic line (1.7 mg/g DW). Also, qRT-PCR analysis showed that the expression levels of two pathway genes was positively correlated with increased accumulation of tanshinone.

Conclusions:

Our study provides an effective strategy for increasing the content of tanshinones and other natural compounds using a combination of genetic engineering and elicitor treatment.

KEYWORDS:

Hairy roots; Salvia miltiorrhiza; SmMDS. elicitors; Tanshinones

Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

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