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Stroke. 2019 Jun;50(6):1590-1594. doi: 10.1161/STROKEAHA.119.024975. Epub 2019 Apr 18.

Extravasation of Microspheres in a Rat Model of Silent Brain Infarcts.

Author information

1
From the Department of Biomedical Engineering and Physics, Amsterdam Cardiovascular Sciences (A.-E.v.d.W., N.L., J.D.V., E.N.T.P.B., E.v.B.), Amsterdam University Medical Center, University of Amsterdam, the Netherlands.
2
Department of Medical Biology, Electron Microscopy Center Amsterdam (A.E.G., N.N.v.d.W.), Amsterdam University Medical Center, University of Amsterdam, the Netherlands.
3
Department of Physiology, Amsterdam University Medical Center, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, the Netherlands (P.L.H.).

Abstract

Background and Purpose- We developed a rat model of silent brain infarcts based on microsphere infusion and investigated their impact on perfusion and tissue damage. Second, we studied the extent and mechanisms of perfusion recovery. Methods- At day 0, 15 µm fluorescent microspheres were injected into the right common carotid artery of F344 rats. At days 1, 7, or 28, the brain was removed, cut in 100-µm cryosections, and processed for immunofluorescent staining and analysis. Results- Injection of microspheres caused mild and transient damage to the treated hemisphere, with a decrease in perfused capillary volume at day 1, as compared with the untreated hemisphere. At day 1 but not at days 7 and 28, we observed IgG staining outside of the vessels, indicating vessel leakage. All microspheres were located inside the lumen of the vessels at day 1, whereas the vast majority (≈80%) of the microspheres were extravascular at day 7, and 100% at day 28. This was accompanied by restoration of perfused capillary volume. Conclusions- Microspheres cause mild and transient damage, and effective extravasation mechanisms exist in the brain to clear microsized emboli from the vessels.

KEYWORDS:

angiophagy; arterioles; brain infarction; cerebrovascular disorders; microcirculation; rats

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