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Stroke. 2019 Jun;50(6):1590-1594. doi: 10.1161/STROKEAHA.119.024975. Epub 2019 Apr 18.

Extravasation of Microspheres in a Rat Model of Silent Brain Infarcts.

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From the Department of Biomedical Engineering and Physics, Amsterdam Cardiovascular Sciences (A.-E.v.d.W., N.L., J.D.V., E.N.T.P.B., E.v.B.), Amsterdam University Medical Center, University of Amsterdam, the Netherlands.
Department of Medical Biology, Electron Microscopy Center Amsterdam (A.E.G., N.N.v.d.W.), Amsterdam University Medical Center, University of Amsterdam, the Netherlands.
Department of Physiology, Amsterdam University Medical Center, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, the Netherlands (P.L.H.).


Background and Purpose- We developed a rat model of silent brain infarcts based on microsphere infusion and investigated their impact on perfusion and tissue damage. Second, we studied the extent and mechanisms of perfusion recovery. Methods- At day 0, 15 µm fluorescent microspheres were injected into the right common carotid artery of F344 rats. At days 1, 7, or 28, the brain was removed, cut in 100-µm cryosections, and processed for immunofluorescent staining and analysis. Results- Injection of microspheres caused mild and transient damage to the treated hemisphere, with a decrease in perfused capillary volume at day 1, as compared with the untreated hemisphere. At day 1 but not at days 7 and 28, we observed IgG staining outside of the vessels, indicating vessel leakage. All microspheres were located inside the lumen of the vessels at day 1, whereas the vast majority (≈80%) of the microspheres were extravascular at day 7, and 100% at day 28. This was accompanied by restoration of perfused capillary volume. Conclusions- Microspheres cause mild and transient damage, and effective extravasation mechanisms exist in the brain to clear microsized emboli from the vessels.


angiophagy; arterioles; brain infarction; cerebrovascular disorders; microcirculation; rats

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