Send to

Choose Destination
Cancer Cytopathol. 2019 May 28. doi: 10.1002/cncy.22139. [Epub ahead of print]

A large series of hyalinizing trabecular tumors: Cytomorphology and ancillary techniques on fine needle aspiration.

Author information

Division of Anatomic Pathology and Histology, Fondazione Policlinico "Agostino Gemelli", IRCCS, Rome, Italy.
Division of Endocrine-Surgery, Fondazione Policlinico "Agostino Gemelli", IRCCS, Rome, Italy.
Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.



Hyalinizing trabecular tumors (HTTs) are rare, essentially benign, follicular cell-derived thyroid neoplasms characterized by a trabecular growth pattern and nuclear pseudoinclusions. Their cytological findings are misleading, because these tumors are often misinterpreted on fine needle aspirate cytology as malignant lesions, such as papillary thyroid cancer and/or medullary thyroid cancer, leading to unnecessary total thyroidectomy. The aim of this study was to analyze the cytomorphological features and application of ancillary techniques in a series of HTTs.


Of 26 histological cases of HTT collected from September 2001 to December 2018, 18 cases had concomitant cytopathology. Cytological cases were processed with liquid-based cytology (LBC). Immunocytochemistry for HBME-1 and galectine-3 as well as molecular testing for BRAFV600E mutation were performed on both LBC and histological specimens.


The 18 lesions with fine needle aspirate cytology ranged in size from 5 to 45 mm. Cytological diagnoses included: 1 benign lesion favoring goiter (5.5%), 4 atypia of undetermined significance (22.2%), 6 follicular neoplasms (33.3%), 5 suspicious for malignancy favoring papillary thyroid cancer (28%), and 2 malignant (11%). Hence, 89% HTT had a negative concordant immunopanel, and they were 100% wild-type BRAFV600E .


The majority of our HTTs (83.3%) were diagnosed in the indeterminate Bethesda categories, suggesting that their cytomorphological features pose issues for reaching a conclusive cytological diagnosis. The ancillary test results in our series support the fact that HTT is a benign neoplasm.


BRAF mutation; hyalinizing trabecular tumor; immunocytochemistry; molecular testing; personalized medicine; thyroid malignancy; thyroid nodule


Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center