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Nat Prod Res. 2019 May 28:1-4. doi: 10.1080/14786419.2019.1608546. [Epub ahead of print]

Unmodified household coffee maker assisted extraction and purification of anticancer agents from Dillenia indica fruits.

Author information

1
a Research Unit , Era's Lucknow Medical College & Hospital, Era University , Lucknow , UP , India.
2
b Department of Biotechnology , Era's Lucknow Medical College & Hospital, Era University , Lucknow , UP , India.
3
c Department of Chemistry , University of Allahabad , Allahabad , UP , India.
4
d Department of Chemistry , Government Raza Post Graduate College , Rampur , UP , India.
5
e Department of Chemistry , Era University , Lucknow , UP , India.

Abstract

Bioassay targeted, 80% aqueous ethanol crude extract of the fruits of Dillenia indica Linn, using the unmodified household coffee maker, afforded five compounds, namely betulinic acid (1), rhamnazin (2), dillenetin (3), luteolin-7-O-β-D-glucoside (4) and hypolaetin-8-O-β-D-glucoside (5). The crude extract, fractions and purified compounds were tested against MDA MB-231, A549 and HeLa cancer cell lines by MTT assay, using betulinic acid 1, as a positive control. Compound 3 showed the best activity against A549 (IC50 = 26.60 ± 2.5 µM) and HeLa cancer cell lines (IC50 =19.35 ± 0.9 µM), whereas compound 5 was found to show the best activity against MDA MB-231 (IC50 = 34.62 ± 5.2µM) cancer cell line. These highly potent anticancer compounds obtained from the fruits of D. indica may be suitable for herbal drug development and formulations.

KEYWORDS:

; anticancer agents; betulinic acid; household coffee maker

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