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Wei Sheng Yan Jiu. 2019 May;48(3):463-467.

[Effects of methionine on the activity of cystathionine-β-synthase].

[Article in Chinese]

Author information

1
National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, The Key Laboratory of Trace Element Nutrition of National Health Commission (NHC), Beijing 100050, China.
2
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Abstract

OBJECTIVE:

To investigate the effects of methionine on the activity of cystathionine-β-synthase.

METHODS:

A total of 56 male rats of the Wistar were randomly divided into 7 groups: 10% casein(10 C) group, 40% casein(40 C) group, 10 C+0.75% L-methionine(10 CM) group, 10 C+amino acid mixture(10 CAA) group, 10 CAA-methionine(10 CAA-Met) group, 10 C+ essential amino acid(10 C+EAA) group, and 10 C+ non-essential amino acid(10 C+NEAA) group, with 8 rats in each group for 10 days.

RESULTS:

The plasma homocysteine concentration significantly increased from(17.1±0.3)μmol/L to(50.7±4.8)μmol/L and(40.5±3.9)μmol/L in rats fed 10 CM and 10 C+EAA diets(P<0.01). Supplementation with methionine induced hyperhomocysteinemia. Compared to 10 C, the activity of hepatic cystathionine-β-synthase(CBS) were significantly increased in the experimental group except for 10 CM(P<0.05). The activity of hepatic CBS was the largest increases in diets with 40 C and the smallest increases in 10 C+NEAA. The activity of hepatic betaine-homocysteine S-methyltransferase(BHMT) were increased in the experimental group except for 10 CAA-Met and 10 C+NEAA(P<0.05).

CONCLUSION:

The increased CBS activity induced by high protein diets is determined by high amino acid intake rather than methionine supplemention.

KEYWORDS:

amino acid; cystathionine-β-synthase; hyperhomocysteinemia; methionine

PMID:
31133135

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