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J Virol Methods. 2019 Aug;270:150-152. doi: 10.1016/j.jviromet.2019.05.009. Epub 2019 May 24.

Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection of Citrus leaf blotch virus.

Author information

1
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
2
College of Life Sciences, Northwest A&F University, 712100, Yangling, Shaanxi, China.
3
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: wuyunfeng@nwsuaf.edu.cn.

Abstract

Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorganisms and exogenous genes. The sensitivity of the RT-LAMP method was increased 100-fold compared to that of the conventional RT-PCR. In addition, this method was fast, simple and specific; it could provide better technical support for field diagnosis, customs quarantine and the control measures of CLBV. To our knowledge, this is the first report detecting CLBV using RT-LAMP.

KEYWORDS:

Citrus leaf blotch virus; Detection; Kiwi fruit; RT-LAMP

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