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Trop Med Int Health. 2019 Aug;24(8):1011-1017. doi: 10.1111/tmi.13273. Epub 2019 Jun 17.

Kelch 13 propeller gene polymorphism among Plasmodium falciparum isolates in Lagos, Nigeria: Molecular Epidemiologic Study.

Author information

1
ANDI Center of Excellence for Malaria Diagnosis, Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria.
2
Chinese Center for Disease Control and Prevention, National Institute of Parasitic Diseases, Shanghai, China.

Abstract

in English, French

OBJECTIVE:

To assess polymorphism in Kelch 13 gene of Plasmodium falciparum isolates in Lagos, Nigeria.

METHODS:

195 Plasmodium falciparum-positive dried blood spots collected from individuals that accessed diagnostic care at some health facilities and during community surveys across several Local Government Areas of Lagos State, Nigeria, were investigated for the presence of mutations in the K13 gene by nested polymerase chain reaction (PCR) using haplotype-specific probes and sequencing.

RESULTS:

Three mutant genotypes of K13 gene were observed: A578S in 0.5%, D464N in 0.5% and Q613H in 1.5%. The frequency of K13 polymorphism was 3.1%, while the remaining parasite population had the wild K13 propeller genes.

CONCLUSION:

No validated Kelch 13 polymorphism associated with artemisinin resistance was seen among P. falciparum isolates from Lagos, Nigeria. As no clinical study was done, this could not be correlated with artemisinin sensitivity.

KEYWORDS:

Plasmodium falciparum ; Kelch 13 gene; artemisinin; artémisinine; gène Kelch 13; resistance; résistance

PMID:
31132213
DOI:
10.1111/tmi.13273

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